Background Information
•Chromatography Basics
Protocols
•Bacterial Transformation
•Bacterial Culture and Expression Induction
•Lysis and Lysate Preparation
•His-Tag Protein Batch Purification
•His-Tag Protein Column Purification
•Ni-Column Stripping and Regeneration
•GST-Tag Protein Column Purification
•Dialysis of Purified Recombinant Protein
•Small Scale Expression Screening
Videos
•MCC Tutorial Expression, Lysis & Purification Background
•MCC Tutorial Ni-His Tag Protein Column Purification
•Dialysis Tutorial
•What is a Plasmid?
•Bacterial Transformation
PPT Files and Handouts
•Instructional PPT (long version) (short version)
•Pre-Lab Quiz
•Purification Homework
•Example of Student Instructions
Helpful Links
•Plasmid 101, Qiagen Expression, GE Health, Novagen
Teacher Tips/FAQ Slack Connection Materials List
Helpful Contact: Joseph Provost josephprovost@sandiego